Development and Validation of Analytical Method for Estimation of Berberine in Herbal Extract of Momordica Dioica

Background:While several anti-diabetic medications have been found to lower blood glucose levels, there are numerous negative effects connected with the use of currently marketed medications.However, medicinal plants low or no side effects. Among Cucurbitaceae family plants, Momordica dioica is a dioecious climbing herb. It contains many phytoconstituents.One it quaternary ammonium salt benzyl-isoquinoline alkaloid called as berberine exhibiting wide variety pharmaceutical properties.In current investigation, activities for its potential in Momorica Dioica plant.The goal recent effort establish and validate an HPTLC method that quick, accurate, exact, specific determining from herbal extract dioica. Methodology/Conclusions/significance: For quick examination determine amount berberine, (HPTLC) process was created well validated.On aluminium plate 60 F254,precoated silica gel chromatographic separation accomplished using solvents: methanol ,ethyl acetate, toluene formic acid (2:4:3:0.5). A wavelength 348 nm used detection. Rf be 0.44±5% berberine. There linearity 400ng/band concentration range.The LOD LOQ be0.0096ng/band 0.0293ng/ band Berberine.The average recovery rate (0.041).The method's precision, accuracy, linearity, robustness, specificity were all authenticated accordance ICH guidelines. The discovered approach can regularly analyse Berberine formulations quality control.